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. Author manuscript; available in PMC: 2009 Sep 3.
Published in final edited form as: Mol Biochem Parasitol. 2009 Jan 22;165(1):67–78. doi: 10.1016/j.molbiopara.2009.01.004

Table 1.

Lipid analysis and composition of the major phospholipids from differentiating Giardia lamblia.a

PL m/z Ion Species Proposed Structure sn-1/sn-2b Relative Abundancec
Positive-Ion Mode
PC 502.4 M + Li lyso-C16:0 +
526.4 M + Li lyso-C18:2 +
528.4 M + Li lyso-C18:1 +
600.5 M + Li C11:0/C11:0 (IS) N/A
740.8 M + Li C16:0/C16:0 +
752.7 M + Li C16:0/C17:1 and/or C18:1/C15:0 +
754.8 M + Li C16:0/C17:1 +
764.8 M + Li C16:1/C18:1 +
766.8 M + Li C16:0/C18:1 ++
768.8 M + Li C16:0/C18:0 +
780.6 M + Li C17:0/C18:1 +
788.6 M + Li C18:2/C18:2 +
790.7 M + Li C18:1/C18:2 +
792.7 M + Li C18:1/C18:1 ++
794.6 M + Li C18:0/C18:1 ++++
806.8 M + Na C18:1/C18:2 +
808.8 M + Na C18:1/C18:1 ++
814.6 M + Li C20:4/C18:1 ++
816.6 M + Li C20:4/C18:0 +
SM 709.9 M + Li C16:0/d18:1 ++
725.7 M + Na C16:0/d18:1 +
737.8 M + Li C18:0/d18:1 +
Negative-Ion Mode
PC 804.6 M + formate C18:1/C16:0 +
806.6 M + formate C18:0/C16:0 +
820.6 M + Cl C18:1/C18:1 +
828.6 M + formate C18:2/C18:1 +
830.6 M + formate C18:1/C18:1 +
832.6 M - H C18:0/C18:1 +
PE 578.4 M - H C12:0/C12:0 (IS) N/A
636.4 M + NaCl - H C12:0/C12:0 (IS) N/A
646.4 M + Na + formate - H C12:0/C12:0 (IS) N/A
714.5 M - H C16:0/C18:2 and/or C16:1/C18:1 ++
716.5 M - H C18:1/C16:0 +++
834.6 M - H C22:6/C22:6 (IS) N/A
PG 609.4 M - H C12:0/C12:0 (IS) N/A
707.5 M - H C16:0/15:0 and/or C14:0/C17:0 +
719.6 M - H C16:0/C16:1 and/or C18:1/C14:0 +
721.5 M - H C16:0/16:0 and/or C18:0/C14:0 +++
733.5 M - H C16:0/C17:1 and/or C18:1/C15:0 +
735.5 M - H C16:0/C17:0 and/or C15:0/C18:0 +
745.5 M - H C18:2/C16:0 and/or C16:1/C18:1 +
747.5 M - H C18:1/C16:0 ++++
749.5 M - H C18:0/C16:0 ++++
761.5 M - H C18:1/C17:0 +
763.6 M - H C18:0/C17:0 and/or C19:0/C16:0 +
771.5 M - H C18:1/C18:2 +
773.5 M - H C18:1/C18:1 +
775.5 M - H C18:0/C18:1 +
777.6 M - H C16:0/20:0 and/or C18:0/C18:0 +
789.5 M + Na + formate – H C16:0/C16:0, C18:0/C14:0, and/or C15:0/C17:0 +
817.5 M + Na + formate – H C18:0/C16:0 +
PI 809.5 M - H C16:0/C16:0 +
835.5 M - H C18:1/C16:0 +
a

Phospholipid species were identified by MS-MS analysis in positive- and negative-ion modes.

b

Relative abundance is designated by the peak height: ++++, up to 100%; +++, up to 75%; ++, up to 50%; +, 10% or less. IS, internal standard.

c

Relative abundances for each sample were similar and therefore only the peak heights for trophozoites are shown.

N/A, not applicable.