Table 3.

Fatty acid analysis by GC-MS.^a

Retention time (min)	Fatty acid	Relative Peak Area (%)b
		Vegetative Trophozoites	0-h Encysting	6-h Encysting	12-h Encysting	24-h Encysting	48-h Encysting	In vitro Cysts	Bovine Serum	Bovine Bile
13.3	C10:0	0.0	0.0	0.0	2.1	0.0	0.0	0.0	0.0	0.0
18.0	C12:0	1.1	1.9	0.0	2.7	0.0	0.7	0.4	1.7	3.2
22.2	C14:0	3.6	3.4	2.7	6.1	0.3	0.8	0.3	2.8	3.2
24.2	C15:0	1.6	2.0	1.3	1.3	0.4	0.6	0.3	2.3	trace
26.3	C16:0	33.5	25.7	32.2	33.3	51.7	38.2	47.7	27.4	30.2
28.6	C17:0	2.6	3.6	2.7	2.0	1.1	0.9	1.5	2.7	2.1
31.1	C18:0	15.9	22.3	39.1	23.4	19.1	45.0	27.6	30.9	22.2
32.2	C18:1	22.7	12.2	10.8	16.1	23.7	9.2	18.2	7.7	31.4
34.4	C18:2	1.3	1.5	2.0	1.8	2.7	2.3	1.3	24.5	5.7
36.2	C20:0	3.5	6.6	2.7	2.7	0.9	1.3	2.1	trace	2.0
37.4	C20:1	0.9	1.4	0.0	0.0	0.0	0.1	0.1	0.0	0.0
38.8	C21:0	1.9	2.7	1.1	1.1	0.0	0.0	0.0	0.0	0.0
41.5	C22:0	3.9	5.6	2.6	2.8	0.1	0.5	0.3	0.0	0.0
44.0	C23:0	2.3	6.2	1.1	1.7	0.0	0.2	0.1	0.0	0.0
46.7	C24:0	4.4	4.9	1.7	2.9	0.0	0.2	0.1	0.0	0.0
48.1	C24:1	0.8	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0
	Total	100	100	100	100	100	100	100	100	100

^aFatty acids present in trophozoites, encysting cells, in vitro-derived cysts, and bovine bile and serum.

^bRelative abundance is designated by peak area and is shown as percentage of the total fatty acid content for each sample.