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. Author manuscript; available in PMC: 2010 Jul 24.
Published in final edited form as: Biochem Biophys Res Commun. 2009 May 12;385(2):143–147. doi: 10.1016/j.bbrc.2009.05.026

Figure 1.

Figure 1

Characterization of the cell line stably expressing FLAG-tagged rat GCH-1. FLAG-GCH-1 HEK 293 cells were stimulated with or without tetracycline (1μg/ml) for 24 hours. (A) Whole cell extracts were analyzed by Western blot analysis for the expression of GCH-1 using anti-FLAG antibody. (B) Cells were lysed for GCH-1 activity assay. (C) Cells were lysed and intracellular BH4 levels were measured by HPLC. Data are expressed as mean±SD (n=3). *P<0.05 vs. the cells without tetracycline treatment.