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Gel retardation assay of poly(disulfide amine)s/DNA polyplexes at varying w/w ratios at the conditions of (A) without and (B) with DTT incubation (10.0 mM, 37°C, 1 h). Lane assignments correspond to polymer/DNA w/w ratios and represented as: lane 1 (0:1, plasmid DNA); lane 2 (bPEI (25kDa)/DNA 1:1); lane 3 (0.1:1); lane 4 (0.2:1); lane 5 (0.5:1); lane 6 (1:1); lane 7 (2:1); lane 8 (5:1); lane 9 (10:1); lane 10 (20:1); lane 11 (30:1).
