Table 1.

Primers utilized in this investigation

Primer name	Primer sequence	Expected product size (bp)
TRPC1 F	5'-GTA AGT GGA TTT GCT CTC AT-3'	298 for TRPC1B splice variant
TRPC1 R	5'-TGG TTA ATT TCT TGG ATA AA-3'
TRPC3 F	5'-TAC TCA ACA TGC TAA TTG CTA TGA T-3'	383
TRPC3 R	5'-CAC AGT TGC TTG GCT CTT GTC TTC C-3'
TRPC4 F	5'-CTC TGG TTG TTC TAC TCA ACA TG-3'	781
TRPC4 R	5'-CCT GTT GAC GAG CAA CTT CTT CT-3'
TRPC5 F	5'-GCT CGC AGC CAC CCC AAA GGG AGG A-3'	548
TRPC5 R	5'-CCA ATG TCC CTA CCC TGT TCT CCC AGC TCT C-3'
TRPC6 F	5'-GAA CTT AGC AAT GAA CTG GCA GT-3'	625 or 277
TRPC6 R	5'-CAT ATC ATG CCT ATT ACC CAG GA-3'
TRPC7 F	5'-GTC CGA ATG CAA GGA AAT CT-3'	477
TRPC7 R	5'-TGG GTT GTA TTT GGC ACC TC-3'
TRPM7 F	5'-AAA CCA GTT CTG CCT CCT CCA C-3'	100
TRPM7 R	5'-GTC CAT CGG AAG TCT TAT CTT TCT TTC-3'
TRPM8 F	5'-GAT TTT CAC CAA TGA CCG CCG-3'	502
TRPM8 R	5'-CCCCAGCAGCATTGATGTCG-3'
TRPV6 F	5'-GAG CAG TCC CTG CTG GAA CT-3'	254
TRPV6 R	5'-GGT ACT TCG AGA CAC TGA GG-3'
Cav3.2 F	5'-GAA GGA CAC GCT GCG CGA GT-3'	180
Cav3.2 R	5'-GCA TCC TCC CGT GCC TCC TT-3'
β-actin F	5'-ATG GAT GAT GAT ATC GCC GC-3'	350
β-actin R	5'-ATC TTC TCG CGG TTG GCC TT-3'
TRPC1 F nested	5'-ATGGCGGCCCTGTACCCGAG-3'
TRPC1 R nested	5'-AACACAATGTGCATTCACAG-3'
TRPC3 F nested	5'-AAGTGACTTCCGTTGTGCTC-3'
TRPC3 R nested	5'-AGGTTGCTGCATCATTCACA-3'
TRPC4 F nested	5'-TGTGACCAATGTCAAAGCAC-3'
TRPC4 R nested	5'-CTAACACACATTGTTCACTG-3'
TRPC5 F nested	5'-CACATCACAACCACGACG-3'
TRPC5 R nested	5'-ACTTGCCTGTAACAGATCGG-3'
TRPC6 F nested	5'-CAA CCA GAA ACA GAA GCA TG-3'
TRPC6 R nested	5'-CTC GCA ATG AAT GAT GCT GC-3'
TRPC7 F nested	5'-CACAGTTCTCCTGGACAGAA-3'
TRPC7 R nested	5'-TTACTTCAGATAAGCCGAAT-3'