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TRH receptor in permeabilized cells. Cells expressing 2HA-AP-TRH receptor and GFP-Rab4, -5, or -11 were treated with 100 nm TRH for 30 sec, fixed, permeabilized, and stained with anti-HA and anti-phospho-TRH receptor antibodies. Overlap of blue HA-TRH receptor and green GFP-Rabs appears cyan in overlay. Bar, 10 μm. TRHR, TRH receptor.
