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. 2009 Jun 18;23(9):1466–1478. doi: 10.1210/me.2009-0119

Figure 3.

Figure 3

TRH receptor internalizes via Rab5-positive endosomes. Cells expressing 2HA-AP-TRH receptor and (A–E) GFP-Rab5 or (F and G) GFP-Rab5-S34N were incubated with anti-HA antibody, stimulated with 100 nm TRH for the indicated times, and fixed and stained. Some cells were washed to remove TRH and allowed to recover for 30 min before fixation. The localization of GFP-Rab5-S34N was quite variable in different cells (e.g. panels F and G and supplemental Fig. 1). Bar, 10 μm. s, Seconds; TRHR, TRH receptor.