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. 2009 Jun 18;23(9):1466–1478. doi: 10.1210/me.2009-0119

Figure 4.

Figure 4

TRH receptor does not colocalize with Rab7 and minimally with Rab11. Cells expressing 2HA-AP-TRH receptor and either GFP-Rab7 (A and B) or GFP-Rab11 (C and D) were incubated with anti-HA antibody and stimulated with 100 nm TRH for the indicated times. Cells in panel E were washed to remove TRH and allowed to recover for 30 min before fixation and staining. Cells expressing 2HA-AP-TRH receptor and constitutively active GFP-Rab11-Q70L (panel F) or dominant-negative GFP-Rab11-S25N (panel G). Bar, 10 μm. s, Seconds; TRHR, TRH receptor.