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. 2009 Jun 18;23(9):1466–1478. doi: 10.1210/me.2009-0119

Figure 5.

Figure 5

Dephosphorylated TRH receptor traffics via Rab4. A–F, Cells expressing 2HA-AP-TRH receptor and GFP-Rab4 were incubated with anti-HA antibody, stimulated with 100 nm TRH for the indicated times, and fixed and stained. Some cells were washed to remove TRH and allowed to recover for the indicated times before fixation. G, Cells expressing 2HA-AP-TRH receptor, GFP-Rab4, and GST-Rab5 were treated as in panel D except that rabbit anti-GST antibody was used to label GST-Rab5. Bar, 10 μm. s, Seconds; TRHR, TRH receptor.