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Arrestin-receptor complex colocalizes with Rab4. Cells expressing 2HA-AP-TRH receptor, GFP-Rab4, and arrestin-mCherry were incubated with anti-HA antibody, stimulated with 100 nm TRH for the indicated times, fixed, and stained. Some cells were washed to remove TRH and allowed to recover for the indicated times before fixation. Bar, 10 μm. Arr-, Arrestin; s, seconds.
