Figure 2.

Dexamethasone dose response for the MR N/C-interaction, alone and in the presence of aldosterone. COS-1 cells were transiently transfected for the M-2-H assay as described in Fig. 1. The cells were treated with various concentrations of dexamethasone alone (A and B) or in the presence of 1 nm aldosterone (C and D) to examine the effect on the MR N/C-interaction with wild-type MRC (A and C) and MRC(E962A) (B and D). Luciferase activity is represented as fold activation relative to luciferase activity in the absence of ligand. Each data point represents the mean ± sem derived from three independent experiments; *, P < 0.001 denotes significance when compared with 1 nm aldosterone in the absence of MRNT (A and B) or when compared with aldosterone alone in the presence of MRNT (C and D).