Figure 6.

Analysis of the role of sumoylation in the MR N/C-interaction. COS-1 cells were transiently cotransfected with GAL4-MRC(E962A) with or without VP16-MRNT (wild type; 1–597) and VP16-MRNT in which the four sumoylation sites have been mutated. Where K1 represents the lysine mutation at position 89, K234 at 399, 428, and 494 and K1234 incorporates the lysine mutations at all four positions. The cells were treated with vehicle (open bars) or aldosterone (10 nm) (solid bars). Luciferase activity is represented as a percentage of the maximal response. Each data point represents the mean ± sem derived from three independent experiments; *, P < 0.001 denotes significance when compared with vehicle.