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GST pull-down assay with the N and C termini. The MR NTD expressed as GST fusion protein, which previously had been coupled to Sepharose glutathione beads, was incubated with [35S]methionine-labeled MR-LBD in the presence (lanes 2) or absence (lane 3) of 1 μm aldosterone. As controls, incubation with SRC-1 (570–780) (positive; lanes 4 and 5), GST alone (lanes 6 and 7) and one tenth of the receptor input (lane 1) are shown. kd, Kilodaltons.
