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. 2009 Sep 22;3(9):e519. doi: 10.1371/journal.pntd.0000519

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Trunck et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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mRNA levels in LSLB without antibiotics-grown late-log cultures of the indicated strains were determined with an amrB-specific primer set. Data were normalized using the 23S rRNA gene as the housekeeping control. amrB transcript levels were determined A in strain 2188a and its gentamicin resistant derivative Bp35 and B in strain 3799a and its gentamicin resistant derivative Bp24. Relative quantifications were performed using 2188a and 3799a, respectively.