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. 2009 Sep 22;3(9):e519. doi: 10.1371/journal.pntd.0000519

Figure 5. Deleted genes are absent from the 708a genome.

Figure 5

PCR was performed with genomic DNA isolated from K strain K96243 or A strain 708a with gene-specific primers. These included 2037 & 2038 for BPSL1801, 2035 & 2036 for BPSL1774, 2033 & 2034 for BPSL1755, 1954 & 1955 for BPSL1743, 2031 & 2032 for BPSL1732, and 1742 & 1743 for BPSL1810. PCR products were separated on a 1% agarose gel and stained with ethidium bromide. Sizes of the expected PCR fragments (in bp and based on K96243 genomic sequence) are indicated above the respective bands. Gene annotations are according to K96243 and gene names, where known, are in parentheses. Lanes M contained the Hi-Lo DNA ladder (Minnesota Molecular, Minneapolis, MN) and the sizes of pertinent fragments are indicated on the left.