Table 3. Antibiotic susceptibilities of B. pseudomallei strains.
Strain | Known Genotype | MIC (µg/ml) for: | |||||
Gma | Str | Spc | Ery | Cla | Cli | ||
1026b | Wild-type | 256 | 1024 | 512 | 128 | 64 | >1024 |
DD503 | 1026b with Δ(amrR-amrAB-oprA) | 2 | NDb | 64 | 8 | 4 | >1024 |
708a | 1 | 8 | 32 | 16 | 16 | >1024 | |
2188a | 1 | 8 | 32 | 16 | 32 | >1024 | |
3799a | 2 | 8 | 64 | 16 | 16 | >1024 | |
Bp24 | Gmr derivative of 3799a | >1024 | 1024 | 256 | 64 | 16 | >1024 |
Bp35 | Gmr derivative of 2188a | >1024 | >1024 | >1024 | 256 | 512 | >1024 |
Bp66 | Low level Gmr derivative of 708a | 32 | 8 | 16 | 4 | 16 | >1024 |
Bp187 | Bp24 with Δ(amrR-amrAB-oprA) | 2 | 16 | 128 | 16 | 16 | >1024 |
Bp202 | Bp187::mini-Tn7T-LACc | 4 | 32 | 128 | 8 | 16 | >1024 |
Bp194 | Bp187::mini-Tn7T-LAC-amrA + B +-oprA +c | >1024 | >1024 | >1024 | 256 | 512 | >1024 |
Bp192 | Bp35 with Δ(amrR-amrAB-oprA) | 2 | 16 | 128 | 16 | 16 | >1024 |
Bp201 | Bp192::mini-Tn7T-LACc | 4 | 32 | 128 | 8 | 16 | >1024 |
Bp200 | Bp192::mini-Tn7T-LAC-amrA + B +-oprA +c | >1024 | >1024 | >1024 | 256 | 256 | >1024 |
Cla, clarithromycin; Cli, clindamycin; Ery, erythromycin; Gm, gentamicin; Spc, spectinomycin; Str, streptomycin.
ND, not done; DD503 is streptomycin resistant because of a chromosomal rpsL mutation.
The mini-Tn7 elements are integrated at the glmS2-associated Tn7 attachment site [20]. MIC values were determined in cells grown in the presence of 1 mM isopropyl-β-D-thiogalactopyranoside.