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. 2009 Sep 15;4(9):e7033. doi: 10.1371/journal.pone.0007033

Figure 4. Knockdown of MRPL28 results in slower growth of tumor cells in vitro.

Figure 4

Figure 4A) Xrystal violet stained colony formation of control scrambled ShRNA and MRPL28 knockdown Miapaca2 cells grown in normoxia and in o.2% oxygen. Figure 4B) Quantitation of plating efficiency shown in figure 4A. Note fewer colonies in normoxia in the knockdown cells, and less of a decrease in moderately severe hypoxic conditions. Figure 4C) Addition of the oxygen radical scavenger NAC does not rescue the growth of the normoxic MRPL28 Miapaca2 knockdown tumor cells. Growth of scrambled control ShRNA cells (closed symbols) and MRPL28 knockdown cells (open symbols). Cell number is reported for growth in control media (dark symbols), or media containing 10 mM NAC (light symbols).