Figure 6. Pharmacologic increase in oxygen consumption results in slower tumor growth.

Figure 6A) SU86 tumors were grown in nude mice and when tumors reached 100–200 mm3, animals were divided into control (PBS) and treated (50 mg/kg DCA IP) groups. Animals were treated daily from day 2 to 16 and tumor volumes measured as indicated. Figure 6B) At day 21 animals were injected with DCA, one hour later by Pimonidozole and 3 hours later sacrifieced. Tumors were explanted and a single cell suspension generated that was stained with FITC-labelled anti-pimonidozole antibody. Cells were analyzed by FACS and the fraction staining >200 (AUs) is indicated.