Fig. 1.

Transcriptomal changes induced by Y27632 in exponentially growing NIH3T3 cells. A Hierarchical cluster diagram of the 289 genes whose expression levels changed in Y27632-treated cells. Each column represents one experiment and each row a gene. Varying levels of expression are represented on a scale from dark blue (lowest expression) to dark red (highest expression). Note that expression values are represented as signal log ratio numbers (SLR) and that, therefore, the total fold change value is obtained from 2^SLR. The experiment number is shown at the top of each column. B Gene graphs showing the induced (red) and repressed (blue) genes in Y27632-treated cells. In each category, the expression values of all deregulated genes are represented as SLR (considering that fold change is 2^SLR; y-axis) obtained in each experimental sample (x-axis). The total number of upregulated and downregulated genes in each category is indicated on the right of each panel. C Histogram showing the number of up- (red) and downregulated (blue) genes with a given expression fold change value in Y27632-treated cells