Figure 5.

Inhibition of angiotensin II (Ang II)-induced O₂⁻ generation in intact PGVSMCs by uncharacterized antioxidants. Cells were pretreated for 2 h with graded concentrations of N-acetyl cysteine (NAC; panel A), (-)-cis-3,3′,4′,5,7-pentahydroxyflavane (2R,3R)-2-(3,4-dihydroxyphenyl)-3,4-dihydro-1(2H)-benzopyran-3,5,7-triol (epicatechin) (panel B), nitroblue tetrazolium (NBT; panel C), ebselen (panel D) or 5,10,15,20-tetrakis (4-sulphonatophenyl) porphyrinate iron (III)(FeTPPS) (panel E), followed by incubation with 10⁻⁶ mol·L⁻¹ Ang II for 4 h. Thereafter, O₂⁻ was measured by inhibition of lucigenin-enhanced chemiluminescence. The values are expressed as mean ± standard error of the mean inhibition, compared with control (0% inhibition, dotted line).