Figure 4.

Effects of oligomycin and carbonylcyanide-p-trifluoromethoxyphenyl hydrazone (FCCP) on mitochondrial tetramethylrhodamine methyl ester (TMRM) fluorescence in flexor digitorum brevis (FDB) fibres isolated from Col6a1^−/− mice treated with vehicle or with Debio 025. Mice were treated for 5 days with vehicle (A) or with 10 mg·kg⁻¹·day⁻¹ Debio 025 (B). FDB fibres were isolated and mitochondrial fluorescence images acquired as described in Methods. Where indicated, 5 µM oligomycin (Oligo) and 4 µM FCCP were added. Each trace shows the fluorescence of a single fibre, and data from all treated animals were pooled.