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. 2009 May 19;157(6):1053–1064. doi: 10.1111/j.1476-5381.2009.00247.x

Figure 2.

Figure 2

RT-PCR analysis of genes showing altered expression in microarray analysis. The level of mRNA transcript was assessed by semi-quantitative RT-PCR analysis. PCR product for each gene was resolved on ethidium-containing agarose gels, quantified by scanning densitometry and normalized to GAPDH level. The values shown are mean ± SD of data from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001 versus untreated control. 15-MPA, 15-methoxypinusolidic acid; ccnd1, cyclin D1; cdc, cell division cycle; cdkn2b, cyclin-dependent kinase inhibitor 2B; ddit3, DNA damage-inducible transcript 3; GADD, growth arrest and DNA damage; ORC, origin–recognition complex; RT-PCR, reverse transcription-polymerase chain reaction.