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SBHA induces accumulation of acetyl-histone H4 (Lys12) (AH4). Total tumor tissue extracts were isolated from SBHA-treated or DMSO-treated mice and analyzed for the levels of AH4. Western blot analysis showed that AH4 is significantly increased in SBHA-treated samples compared to those in the control group. G3PDH was used to confirm equal protein loading. The quantification of the bands was generated by comparison with G3PDH.
