Table 1.
Parameter | FMO1 | FMO3 |
---|---|---|
Km, mM | 3.0 ± 0.5 | 3.4 ± 0.8 |
Vmax, pmol/min/mg | 21.3 ± 2.0 | 42.5 ± 5.9 |
Vmax, pmol/min/pmol | 0.025 ± 0.002 | 0.044 ± 0.006 |
Kinetic constants were determined based on metabolism experiments with voriconazole (1 µM-8000 µM) and recombinant human FMO enzymes (60 pmol/mL) for 30 minutes in the presence of NADPH. The Michaelis-Menten equation was fitted to the rates of N-oxide formation using non-linear regression approach in WinNonLin software v.4.