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. Author manuscript; available in PMC: 2009 Sep 4.
Published in final edited form as: Drug Metab Dispos. 2008 Mar 24;36(6):1119–1125. doi: 10.1124/dmd.107.019646

Table 1.

Kinetic Constants for Voriconazole Metabolism by Recombinant Human Flavin-containing Monooxygenases

Parameter FMO1 FMO3
Km, mM 3.0 ± 0.5 3.4 ± 0.8
Vmax, pmol/min/mg 21.3 ± 2.0 42.5 ± 5.9
Vmax, pmol/min/pmol 0.025 ± 0.002 0.044 ± 0.006

Kinetic constants were determined based on metabolism experiments with voriconazole (1 µM-8000 µM) and recombinant human FMO enzymes (60 pmol/mL) for 30 minutes in the presence of NADPH. The Michaelis-Menten equation was fitted to the rates of N-oxide formation using non-linear regression approach in WinNonLin software v.4.