Skip to main content
. Author manuscript; available in PMC: 2009 Sep 4.
Published in final edited form as: Circ Res. 2008 May 1;102(10):1182–1191. doi: 10.1161/CIRCRESAHA.107.167080

Figure 4. PTP1B overexpression inhibits while PTP1B knockdown enhances VEGF-induced phosphorylation of ERK1/2, but not p38MAPK.

Figure 4

Figure 4

HUVECs infected with Ad.LacZ (control) or Ad.PTP1B-WT (A) or transfected with scrambled or PTP1B siRNAs (B) were stimulated with VEGF (20 ng/mL) for 5 min. Lysates were immunoblotted with phospho-ERK1/2, ERK1/2, phospho-p38MAPK, p38MAPK, PTP1B or actin antibody. Representative blots for ERK1/2 and actin are reprobe from p-ERK1/2 membrane. The representative p-p38MAPK blot is derived from different lysates used for p-ERK1/2 blot, and is reprobed for p38MAPK blot. Bottom panels show averaged data obtained from more than 3 independent experiments, expressed as fold change of phosphorylation over basal (means ± S.E., n=3). *P < 0.05 vs. Ad.LacZ (A) or Scrambled siRNA (B) at each time point.