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. Author manuscript; available in PMC: 2009 Sep 4.
Published in final edited form as: Eur J Immunol. 2009 Jun;39(6):1652–1663. doi: 10.1002/eji.200838135

Figure 3.

Figure 3

IL-4 has a critical role in the development of experimental anesthetic DILI. Representative H & E histological section from (A) WT and (B) IL-4−/− (KO) mice demonstrating significantly more hepatitis in WT mice (A and B, 0.5µm, representative data, 64x magnification). (C) Composite injury scores in WT and KO mice showed increased hepatitis in WT when compared to KO mice (**p<0.01, Mann-Whitney U. Experiments were performed in duplicate with N=5 mice/group). (D) Sera analysis for TFA and S100 antibodies showed significantly elevated TFA IgG1 antibodies as well as anti-S100 IgG1 and IgG2a antibodies in WT compared to KO mice (***p<0.001, ANOVA with Tukey’s post test. Mean ± SEM of pooled data from individual mice. Experiments were performed in duplicate with N=5 mice/group). (E, F) Analysis of cytokines in liver tissue supernatants showed that WT mice had significantly elevated levels of IL-1β, IL-4, IL-5, IL-6, IL-12p70, IL-13 and TNF-α when compared to KO mice (*p<0.05, **p<0.01, ***p<0.001, Mann-Whitney U. Mean ± SEM of pooled data from individual mice. Experiments were performed in duplicate with N=5 mice/group ).