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. Author manuscript; available in PMC: 2010 Jun 12.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Apr 18;383(4):497–502. doi: 10.1016/j.bbrc.2009.04.049

Fig. 1. Exogenous expression of (A) FLAG-eIF5A and (B) GFP-eIF5A precursors or their hypusine-modified forms.

Fig. 1

HeLa cells were transfected with 3XFLAG-CMV-7.1, 3XFLAG-CMV-7.1/heIF5A-1, pCEFL, or pCEFL/GFP-heIF5A-1 with or without pCEFL/hDHS and pCEFL/hDOHH as indicated. Transfection was performed in parallel in two sets, one set of cells (6 well dishes) were used for western blotting using an eIF5A antibody (BD Bioscience) that detects eIF5A(Lys), eIF5A(Dhp) and eIF5A(Hpu) equally well. The other set (60 mm dishes) was used for labeling with [3H]spermidine (5 μCi/ml). Cells were harvested after 42 h of transfection.