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. Author manuscript; available in PMC: 2010 Jun 12.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Apr 18;383(4):497–502. doi: 10.1016/j.bbrc.2009.04.049

Fig. 2. Identification of the major acetylation site of eIF5A as Lys47.

Fig. 2

HeLa cells were transfected as indicated and harvested after 48 h of transfection. 10 μg of proteins was used for western blotting using either AcLys antibody (Santa Cruze Biotechnology), eIF5A antibody (BD Bioscience), or DHS and DOHH rabbit polyclonal antibodies.