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. Author manuscript; available in PMC: 2010 Jun 12.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Apr 18;383(4):497–502. doi: 10.1016/j.bbrc.2009.04.049

Fig. 4. Comparison of the cellular distribution of GFP-eIF5A mutant proteins.

Fig. 4

(A) direct visualization of GFP fluorescence after 48h of transfection. HeLa cells were transfected with vectors encoding GFP-eIF5A wt and mutants, without (upper panels) or with (lower panels) vectors encoding DHS and DOHH. (B) western blots of the above samples using AcLys and eIF5A antibodies (BD Bioscience).