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. Author manuscript; available in PMC: 2009 Sep 4.
Published in final edited form as: J Cell Biochem. 2009 Feb 1;106(2):306–316. doi: 10.1002/jcb.22007

Figure 3. LMHF mechanical loading induced mineralization in a magnitude-dependent manner.

Figure 3

Confluent 2T3 cells were exposed to Static, Static+BMP4, or Static+LMHF conditions at varying loading magnitudes (0.1-0.4g for 10 min/day) for 21 days and stained for calcium deposition using Alizarin Red. The Opticell membrane was scanned (A), and quantification (B) was determined using imaging software and graphed as a % of static control. Data are expressed as mean ± SEM (n=6-10, *p<0.01, **p<0.05).