Confluent 2T3 cells were exposed to Static, Static+BMP4, or Static+LMHF conditions at varying loading magnitudes (0.1-0.4g for 10 min/day) for 21 days and stained for calcium deposition using Alizarin Red. The Opticell membrane was scanned (A), and quantification (B) was determined using imaging software and graphed as a % of static control. Data are expressed as mean ± SEM (n=6-10, *p<0.01, **p<0.05).