2T3 cells were exposed to Static, Static+LMHF, RPM, RPM+LMHF, and RPM+Static conditions for 3 days. LMHF was applied to the appropriate groups at 0.3g (A) or 0.4g (B) for 10 min/day. ALP activity was measured using a colormetric assay, normalized to total protein, and graphed as a % of static control. Data are expressed as mean ± SEM (n=6-9 *p<0.01, **p<0.05).