Confluent 2T3 cells were exposed to Static, Static+LMHF, RPM, and RPM+LMHF conditions for 3 days. LMHF loading was applied to the appropriate groups at 0.3g for 10 min/day. Total RNA was obtained from the cell lysates, purified, and reverse transcribed to obtain cDNA. Quantitative real time PCR was performed for ALP (A), runx2 (B), osteomodulin (C), parathyroid hormone receptor 1 (D), and osteoglycin (E) using 18s rRNA as an internal control. Data are expressed as mean ± SEM (n=3-10, *p<0.05).