Figure 10. Seeding with oligomers or fibrils (total insulin concentrations were not kept constant).

Two different data sets are shown here in terms of absolute absorbance readings, A₆₀₀, versus time, t. For the first set, different amounts of dissolved fresh insulin at 40 mg (), 30 mg (), 20 mg (), 10 mg () and 0 mg (×) (control experiment) were added to the same amount (∼ 2 mg) of pre-washed fibrils, collected at the end of a previous fibrillation run. Fits () were obtained using Eq. (2) from the empirical model. For the second set, the same washed fibrils were added to new pre-washed fibrils, collected at different times during the elongation phase at times: 3.5 h (○), 3.8 h (△) and 4 h (◇) or at 15, 34, and 50 % of the A_600,asym values, respectively. All the samples were placed at 65±2°C and pH 1.6. For each run 8 mg of fibers were resuspended in 20 ml of fresh buffer.