Figure 8. Seeding experiments (with oligomers).

Samples comprising 2% by volume were taken at increasing times, t_ext,i, during an insulin fibrillation run prior to the formation of fibrils and added as seeds to fresh new insulin runs (total insulin concentration was kept at 2 mg/ml, 65±2°C and pH 1.6). Sigmoidal plots of the fibrillation process for A₆₀₀ versus time, t, in hours. Reference data (no seeds added) (◆) and data with seeds extracted at t_ext,i of 0.5 h (), 1 h (), 2 h (), 2.5 h (), 3 h (). Fits () were obtained using Eq. (2) from the empirical model.