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. Author manuscript; available in PMC: 2010 Oct 1.

Published in final edited form as: Proteins. 2009 Oct;77(1):62–73. doi: 10.1002/prot.22417

Parameters resulting from a best fit of Eq. (2) to the data in Fig. 7: (a) t_lag, (b) A_600,asym and (c) k_app as a function of the extraction time, t_ext,i. Reference data (without seeding) (◆) and seeding experiments using 2% v/v (, and , respectively) and 10% v/v (, and , respectively) seeds (total insulin concentration was kept at 2 mg/ml, 65±2°C and pH 1.6). The linear fits (), t_lag = −0.831x + 2.652, (R²=0.923) and A_600,asym = 0.138t_ext + 0.988, (R²=0.917). The relative decrease in t_lag, and increase in A_600,asym after 3 hours were 93% and 24%, respectively.

Inline graphic — Parameters resulting from a best fit of Eq. (2) to the data in Fig. 7: (a) t_lag, (b) A_600,asym and (c) k_app as a function of the extraction time, t_ext,i. Reference data (without seeding) (◆) and seeding experiments using 2% v/v (, and , respectively) and 10% v/v (, and , respectively) seeds (total insulin concentration was kept at 2 mg/ml, 65±2°C and pH 1.6). The linear fits (), t_lag = −0.831x + 2.652, (R²=0.923) and A_600,asym = 0.138t_ext + 0.988, (R²=0.917). The relative decrease in t_lag, and increase in A_600,asym after 3 hours were 93% and 24%, respectively.