HDAC inhibition by SFN-rich broccoli sprouts in human volunteers. Three healthy human volunteers refrained from cruciferous vegetable intake for 48 h. Each subject consumed 68 g broccoli sprouts (∼105 mg SFN) with a bagel and cream cheese, and blood was collected at times indicated. PBMCs were immunoblotted for acetylated histones H3 and H4 (acH3, acH4), or the corresponding total histone (H3, H4), and HDAC activities were determined using the BioMol kit. Error bars indicate mean±S.E.; *P < 0.05, n=3. For further details, see Ref. [18].