Table 2.

The number (mean ± SEM) of ChAT+, pCREB+, c-Fos+, ChAT+/pCREB+, and ChAT+/c-Fos+ cells in the pedunculopontine tegmentum nucleus (PPT) and laterodorsal tegmental nucleus (LDT) in control REM sleep (CR), low REM sleep (LR), and high REM sleep (HR) groups (n = 7 animals/group)

Brain Area & I. R.	CR (n =7)	LR (n =7)	HR (n =7)	One-Way ANOVA
PPT
ChAT+	97.86 ± 6.62	97.14 ± 5.7	103.0 ± 5.9	F = 0.297; p = 0.76
pCREB+	54.29 ± 3.99	14.86 ± 1.91***	96.57 ± 5.19*** +	F = 107.7; p<0.001
ChAT+/pCREB+	38.43 ± 3.02	8.43 ± 1.73***	92.14 ± 5.22*** +	F = 137.2; p<0.001
PPT
ChAT+	101.6 ± 5.10	104.0 ± 7.50	103.9 ± 6.40	F = 0.045; p = 0.95
c-Fos+	12.14 ± 1.24	5.71 ± 1.19*	64.0 ± 3.91*** +	F = 167.9; p<0.001
ChAT+/c-Fos+	8.57 ± 1.27	2.43 ± 0.43***	48.86 ± 4.03*** +	F = 106.0; p<0.001
LDT
ChAT+	114.1 ± 4.94	118.7 ± 6.69	119.9 ± 5.36	F = 0.280; p = 0.76
pCREB+	19.43 ± 2.27	25.57 ± 3.10	22.86 ± 3.35	F = 0.261; p<0.65
ChAT+/pCREB+	10.29 ± 1.17	15.43 ± 1.88	14.43 ± 1.69	F = 2.880; p<0.85
LDT
ChAT+	117.7 ± 5.55	118.9 ± 6.25	118.3 ± 5.40	F = 0.009; p = 0.99
c-Fos+	10.57 ± 1.13	7.25 ± 1.35	8.50 ± 0.76	F = 0.108; p = 0.75
ChAT+/c-Fos+	5.29 ± 0.61	4.43 ± 0.53	4.29 ± 0.52	F = 0.010; p = 0.81

Bilateral cell counts for each nucleus were taken from three adjacent series of sections cut at 25 μm and collected at 100 μm intervals. The total number of labeled (+, immunoreacted, I.R.) cells in each nucleus of each rat was counted in six boxes (300 × 300 μm) placed on the center of the nucleus. Significant differences with respect to CR is indicated by

^*p<0.05

^***p<0.001

and with respect to LR by

⁺p<0.001