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. Author manuscript; available in PMC: 2009 Sep 4.
Published in final edited form as: J Biol Chem. 2001 May 29;276(32):30442–30451. doi: 10.1074/jbc.M102342200

FIG. 6. Apg2 co-localized with the Apg9 compartment.

FIG. 6

The wild type (SEY6210) strain transformed with the APG9 2µ plasmid was grown in SMD to A600 = 1.0, converted to spheroplasts, and osmotically lysed. Crude cell lysates were pre-cleared, and centrifuged to obtain a total membrane pellet by centrifugation at 100,000 × g for 20 min. Pellets were resuspended in lysis buffer and loaded on an OptiPrep density gradient ranging from 0 to 66%, and centrifuged for 16 h at 100,000 × g as described under “Experimental Procedures.” A total of 14 fractions were collected from the top of the gradient and examined by immunoblot with antibodies or antiserum against A, Pho8 (vacuole), Dpm1 (endoplasmic reticulum), Mnn1 (Golgi), Pep12 (endosome), and Apg2; and B, Apg2 and Apg9.