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. Author manuscript; available in PMC: 2010 May 8.
Published in final edited form as: Cell Host Microbe. 2009 May 8;5(5):439–449. doi: 10.1016/j.chom.2009.04.006

Fig. 4. Replication and pathogenicity of recombinant influenza viruses.

Fig. 4

(A) Multicycle replication of recombinant influenza viruses in A549 cells infected at an MOI of 0.001 pfu per cell. (B) Virus production from interferon-deficient 8-day old embryonated chicken eggs at 24 h post-infection with 100 pfu of each virus per egg. (C) Percent body weight loss of mice following virus infection. Female Balb/C (n = 5) were infected intranasally with 1x104 pfu of one of the indicated viruses or mock-infected with PBS. Body weights were measured for 12 consecutive days following infection. (D) Kaplan-Meier survival curves of mice infected with the indicated recombinant viruses. (E) Viral pulmonary titers of infected mice determined on day 3 and 5 post-infection (n = 3) by plaque assay. Asterisk indicates ΔNS1 not detected by plaque assay. Bars indicate standard deviation. (F) IFN bioassays measured IFN production by influenza virus-infected A549 cells. Fluorescence units represent the extent of NDV-GFP replication in Vero cells treated with supernatants from virus-infected A549 cells. Bars indicate standard deviation. The color scheme for the indicated viruses is provided at the bottom of the figure.