TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| M. bovis BCG | ||
| Pasteur 1173 | Wild type | Laboratory collection |
| KOP55 | Pasteur p55::Ωhyg | This study |
| KOP55 PAZ23 | Strain KOP55 containing pPAZ23 | This study |
| PAZ23 | Strain Pasteur 1173 containing pPAZ23 | This study |
| Plasmids | ||
| pGEM-T Easy | E. coli cloning vector, ampicillin resistance | Promega |
| pIJ2925 | Cloning vector, pUC18 derivative with BglII sites flanking MCS,a ampicillin resistance | 16 |
| pSUM series | E. coli-mycobacteria shuttle vectors, nontranscribed MCS, kanamycin resistance | 2 |
| p2NIL | Gene manipulation mycobacterial suicide vector, kanamycin resistance | 27 |
| pGOAL17 | PAg85-lacZ Phsp60-sacB PacI cassette vector, ampicillin resistance | 27 |
| pHP45-Ωhyg | Ωhyg, ampicillin resistance | 31 |
| pSAN6 | p27-p55 cloned into pIJ2925 | This study |
| pPAZ23 | Pp27-p55-p27-p55 cloned into pSUM41 | 39 |
| pILI10 | p27-p55 cloned into p2NIL | This study |
| pILI11 | p27-p55::Ωhyg cloned into p2NIL | This study |
| pILI12 | p27-p55::Ωhyg cloned into p2NIL with pGOAL17 PacI cassette | This study |
a
MCS, multicloning site.