TABLE 3.

Antimicrobial susceptibilities of M. bovis BCG P55 strains

Compound	MIC (mg/liter) for M. bovis BCG straina:
	Wild type	KOP55b	KOP55 PAZ23c	PAZ23d
Bacitracin	>256	16	>256	NDf
Bacitracin + CCCPe	>256	16	>256
CCCP	4	2	ND	ND
Clofazimine	0.1	0.025	0.1	0.1
Chlorpromazine	4	4	ND	ND
Econazole	6.4	1.6	6.4	12.8
Ethambutol	4	2	ND	ND
Novobiocin	16	2	16	ND
Novobiocin + CCCPe	4	1	4
Novobiocin + VALe	8	0.5	8
Novobiocin + RPe	16-8	1	16-8
PA-824	0.25	0.125	0.25	ND
Reserpine	2	2	2	ND
Rifampin	0.0032	0.0004	0.0032	0.0032
Rifampin + CCCPe	0.0008	0.0002	0.0008
Rifampin + VALe	0.0016	0.0002	0.0016
Rifampin + RPe	0.0032-0.0016	0.0004	0.0032-0.0016
Valinomycin	0.4	0.2	0.4	ND
Vancomycin	4	0.25	4	8
Vancomycin + CCCPe	4	0.25	4
Triclosan	8	8	ND	ND

^aMICs were assayed over a range of twofold dilutions of antibiotics. The MICs of clofazimine, chlorpromazine, econazole, and triclosan were determined on agar plates. The MICs of bacitracin, CCCP, ethambutol, novobiocin, reserpine, rifampin, valinomycin, and vancomycin were determined by the resazurin assay. No difference in susceptibility between wild-type and KOP55 strains was observed for the following compounds: acriflavine, amikacin, cerulenin, d-cycloserine, ethidium bromide, ciprofloxacin, chloramphenicol, ethionamide, fluconazole, gentamicin, imipenem, isoniazid, ofloxacin, p-aminosalicylic acid, streptomycin, spectinomycin, and tetracycline (data not shown).

^bKOP55 is M. bovis BCG with the p55 gene inactivated.

^cKOP55 PAZ23 is complemented M. bovis BCG KOP55 with plasmid pPAZ23 (p27-p55 operon cloned into pSUM41).

^dPAZ23 is M. bovis BCG containing plasmid pPAZ23 (p27-p55 operon in pSUM36).

^eCCCP, valinomycin (VAL), and reserpine (RP) were present at concentrations of 1, 0.1, and 0.5 mg/liter, respectively (fourfold the sub-MIC for the M. bovis BCG wild type).

^fND, not determined.