TABLE 4.
Susceptibilities of M. bovis BCG P55 strains to redox compounds
| Redox compounda | Disc load (μmol) | Inhibition zoneb (mm) for M. bovis BCG strains
|
|
|---|---|---|---|
| Wild type | KOP55c | ||
| Hydrogen peroxide | 80 | 23 | 23 |
| dl-Dithiothreitol | 5 | 0 | 30d |
| l-Glutathione reduced | 10 | 0 | 0 |
| Diamide | 5 | 0 | 30 |
| Menadione | 0.125 | 20 | 16 |
| mBBre | 0.5 | 0.125-0.25 | |
a
Dimethyl sulfoxide, which was used as the solvent for some of these compounds, had no effect on the growth of either strain (data not shown).
b
Halos were recorded after 14 days at 37°C.
c
KOP55 is M. bovis BCG with the p55 gene inactivated.
d
Delayed growth inside the halo.
e
Susceptibility to monobromobimane (mBBr) was determined by the Resazurin assay, and the concentrations are shown as the MICs (mM) for M. bovis BCG strains.