FIG. 1.

Structure of ALN-RSV01 duplex and IC₅₀ of ALN-RSV01 in vitro. (A) ALN-RSV01 is a synthetic double-stranded RNA oligonucleotide formed by hybridization of two partially complementary single-stranded RNAs in which the 3′ ends are capped with two thymidine units. (B) Vero cells in 24-well plates were transfected with decreasing concentrations of ALN-RSV01, followed by infection with 200 to 300 PFU of RSV A2. At 5 days postinfection, the cells were fixed, immunostained, and counted. The activity of ALN-RSV01 was plotted as a percentage of that of PBS, and the IC₅₀ was measured with XLFit software. Error bars represent standard deviations.