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. 2009 Jun 8;53(9):3952–3962. doi: 10.1128/AAC.00014-09

FIG. 6.

FIG. 6.

TNF-α- and IFN-α-stimulatory mismatched siRNAs do not modulate RSV in vivo. (A) Transfection reagent alone (Txn) or siRNAs (AL-DP-5048 [5048] and AL-DP-1730 [1730]) transfected into PBMCs were assayed for TNF-α stimulation at 24 h posttransfection. (B) siRNAs (AL-DP-5048 [5048] and AL-DP-2153 [2153]) transfected into PBMCs were assayed for IFN-α stimulation at 24 h posttransfection. (C) Lung virus concentrations from mice dosed i.n. with RSV at day 0 and with RSV-specific siRNAs (ALN-RSV01) or mismatch control immunostimulatory siRNAs (AL-DP-2153 [2153] and AL-DP-1730 [1730]) at 4 h before inoculation. Lung RSV concentrations were measured by an immunostaining plaque assay at day 5 postinfection.