FIG. 8.

ALN-RSV01 viral inhibition is mediated by RNAi in vivo. Shown is a schematic representation of the 5′ RACE assay used to demonstrate the generation of the site-specific cleavage product. Boxed are the results of sequence analysis of individual clones from PCR amplification of cDNAs generated from linker-adapted RSV N-gene mRNA isolated from an in vivo viral inhibition assay in which mice were inoculated with RSV at day 0 and treated with ALN-RSV01, AL-DP-2153 (Neg Control), or PBS at day 3, followed by lung homogenization and evaluation by RACE at day 5 postinfection.