TABLE 1.
QRDR mutations, PMQR genes, and MICs of antimicrobial agents for ten non-classical phenotype S. enterica strains, their corresponding transformants and seven control strains
| Isolate no.a | Serovar | Year of isolation | parC QRDR mutationb | PMQR genec
|
Plasmid size (kb) (est)d | MICs (μg/ml) of antimicrobial agents for:e
|
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| qnrA | qnrB | qnrS | qnrD | qepA | aac(6′)- Ib-cr | Isolates
|
Transformants
|
||||||||||||
| NAL | NOR | OFX | ENR | CIP | LVX | MFX | NAL | CIP | |||||||||||
| s2017 | S. Corvallis | 2003 | Thre57→Ser | − | − | + | − | − | − | 13 | 16 | 2 | 2 | 2 | 0.25 | 1 | 2 | 8 | 0.25 |
| s2018 | S. Stanley | 2003 | Thre57→Ser | − | − | + | − | − | − | ND | 16 | 2 | 2 | 1 | 0.5 | 1 | 2 | ND | ND |
| s2052 | S. Corvallis | 2003 | Thre57→Ser | − | − | + | − | − | − | 13 | 16 | 1 | 1 | 1 | 0.5 | 0.5 | 2 | 8 | 0.25 |
| s2093 | S. Mbandaka | 2003 | Thre57→Ser | + | − | − | − | − | − | 14.5 | 16 | 16 | 1 | 0.5 | 4 | 1 | 0.5 | ND | ND |
| s2209 | S. Montevideo | 2004 | Thre57→Ser | − | − | + | − | − | − | 30 | 32 | 2 | 2 | 2 | 0.5 | 1 | 2 | 8 | 0.25 |
| s2219 | S. Virginia | 2004 | Thre57→Ser | − | − | + | − | − | − | 13 | 32 | 2 | 2 | 2 | 0.5 | 1 | 2 | 8 | 0.5 |
| s2425 | S. Typhimurium | 2005 | WT | − | − | + | − | − | − | 13 | 16 | 2 | 2 | 2 | 0.5 | 1 | 2 | 8 | 0.5 |
| s2705 | S. Typhimurium | 2006 | WT | − | − | + | − | − | − | 13 | 16 | 2 | 2 | 2 | 0.5 | 1 | 2 | 8 | 0.5 |
| s2856 | S. Braenderup | 2007 | Thre57→Ser | − | − | + | − | − | − | 15 | 16 | 2 | 2 | 2 | 0.5 | 1 | 2 | ND | ND |
| s2944 | S. Montevideo | 2007 | Thre57→Ser | − | − | + | − | − | − | ND | 16 | 2 | 2 | 2 | 0.5 | 1 | 2 | 8 | 0.25 |
| s2043 | S. Stanley | 2003 | Thre57→Ser | − | − | − | − | − | − | ND | 4 | 0.125 | 0.125 | 0.06 | 0.015 | 0.06 | 0.06 | ND | ND |
| s2064 | S. Corvallis | 2003 | Thre57→Ser | − | − | − | − | − | − | ND | 4 | 0.06 | 0.03 | 0.03 | 0.015 | 0.06 | 0.06 | ND | ND |
| s2159 | S. Mbandaka | 2003 | Thre57→Ser | − | − | − | − | − | − | ND | 4 | 0.125 | 0.125 | 0.06 | 0.008 | 0.06 | 0.125 | ND | ND |
| s2317 | S. Montevideo | 2004 | Thre57→Ser | − | − | − | − | − | − | ND | 4 | 0.125 | 0.125 | 0.06 | 0.015 | 0.06 | 0.06 | ND | ND |
| s2878 | S. Typhimurium | 2007 | WT | − | − | − | − | − | − | ND | 16 | ND | ND | ND | 0.03 | ND | ND | ND | ND |
| s2906 | S. Braenderup | 2007 | Thre57→Ser | − | − | − | − | − | − | ND | 4 | ND | ND | ND | 0.015 | ND | ND | ND | ND |
| SL1344 | S. Typhimurium | WT | − | − | − | − | − | − | ND | 4 | ND | ND | ND | 0.06 | ND | ND | ND | ND | |
a
Test strains are isolate numbers s2017 to s2944, and the control strains are s2043 to SL1344.
b
WT, wild-type allele (no mutation).
c
+, present; −, absent.
d
est, estimated; ND, not determined.
e
NAL, nalidixic acid; NOR, norfloxacin; OFX, ofloxacin; ENR, enrofloxacin; CIP, ciprofloxacin; LVX, levofloxacin; MFX, moxifloxacin. MICs tested with standard agar plate dilution method.