FIG. 4.

Effects of D21-6076 on β-1,6-glucan synthesis. (A) C. albicans ATCC 90028 was cultured in MOPS-buffered RPMI containing [¹⁴C]glucose with or without D21-6076. After 3 h of treatment at 30°C, cells were harvested, and β-1,3-glucan, β-1,6-glucan, chitin, and mannan fractions were prepared by the methods described in Materials and Methods. The percent changes of the incorporated radioactivities by drug treatment are displayed. (B) The percent reductions of the incorporation of [¹⁴C]glucose into β-1,6-glucan fractions in growing cells of C. albicans ATCC 90028, C. glabrata ATCC 48435, and C. krusei ATCC 44507 by the treatment of D21-6076 were compared.