TABLE 1.
S. cerevisiae strains used in this study
| Strain | Genotype | Source/reference |
|---|---|---|
| YPH500 | matα ade2 his3 leu2 lys2 trp1 ura3 | 28 |
| AY-10c | Δskn1::URA3 ade2 his3 lys2 in YPH500 background | Kitamura et al., U.S. patent application 20040091949; international patent application PCT/JP01/03630 (2003) |
| CY-1aa | Δkre6::HIS3 pUAE1 (ScKRE6) in AY-10c | This study |
| CY-3aa | Δkre6::HIS3 pUAE3 (ScKRE6-CaSKN2) in AY-10c | This study |
| CY-4aa | Δkre6::HIS3 pUAE4 (ScKRE6-CaKRE6) in AY-10c | This study |
| CY-5aa | Δkre6::HIS3 pUAE5 (ScKRE6-CaSKN1) in AY-10c | This study |
a
The strains were constructed by the methods illustrated in Fig. 2. CY-1a, CY-3a, CY-4a, and CY-5a are designed to express ScKre6p or the fusion protein of ScKre6p and C. albicans Skn2p, Kre6p, or Skn1p, respectively, under the promoter of ScKRE6.