TABLE 2.

Primers used in this study

Primer	Gene	Purpose	Direction	Sequence
SCKRE6-Sen3	ScKRE6	PCR	Forward	5′-CGCGGCCGTAACAAAACGAACAACATGAGACAAAACCCG-3′
SCKRE6-Anti3	ScKRE6	PCR	Reverse	5′-CGAGGCCTTTAGTTCCCTTTATGACCCGATTTGAAC-3′
CASKN1-Sen2	CaSKN1	PCR	Forward	5′-GCGGATCCTGATACTCCTGAAGATG-3′
CASKN1-Anti2	CaSKN1	PCR	Reverse	5′-GCGGCGCCTAAATATAGGGGGGTTGGTGTTTT-3′
CAKRE6-Sen4	CaSKN2	PCR	Forward	5′-GCGGATCCGGATACTCCACAGGACGC-3′
CAKRE6-Rev2	CaSKN2	PCR	Reverse	5′-CCTTCAAATATCATACAC-3′
SCKRE6-Sen3	ScKRE6	PCR	Forward	5′-CGCGGCCGTAACAAAACGAACAACATGAGACAAAACCCG-3′
SCKRE6-Anti4	ScKRE6	PCR	Reverse	5′-CGGGATCCACCAGAGATGTTCTAATGGC-3′
CAKRE6h-MP9	CaKRE6	Mutagenesis		5′-GGTCATTTAGAAATTAGCGCTCGTTTACCAAATTATGG
CASKN1-MP1	CaSKN1	Mutagenesis		5′-GGGGAAATTGGAATTTAGCGCAAAATTACCCGG-3′
CASKN1-MP2	CaSKN1	Mutagenesis		5′-CCAGGGTATCTTGCCAGTACTGAAGGTGTTTGGC-3′