TABLE 1.

Isogenic insertional mutants of M. smegmatis with known defects in cell envelope composition and/or biocide susceptibility

Strain	Mutation	Description/phenotypea	Reference
mc2155ΔpimE	pimE	KO mutant deficient in polar PIM synthesis	Our work
mc2155ΔMSMEG4245	MSMEG_4241	KO mutant deficient in the production of WT LM and LAM; produces a truncated form of LM	14
mc2155ΔMSMEG4250	MSMEG_4247	KO mutant deficient in the production of LM; produces a truncated form of LAM lacking α-1,2 Manp branches on the mannan core	13
mc2155ΔembA	embA	KO mutant deficient in synthesis of the terminal hexa-arabinofuranoside motif of arabinogalactan; decreased cell wall-bound mycolic acid content	9
mc2155ΔembB	embB	KO mutant deficient in synthesis of the terminal hexa-arabinofuranoside motif of arabinogalactan; decreased cell wall-bound mycolic acid content	9
mc2155ΔembC	embC	KO mutant deficient in LAM synthesis	32
mc2155Δlsr2 (DL2008)	lsr2	KO mutant deficient in the regulatory histone-like protein Lsr2	2
MN01	mspA	KO mutant deficient in the production of the major porin MspA	27
ML10	mspA-mspC	KO mutant deficient in the production of the MspA and MspC porins	28
Myc55	MSMEG_0408	Transposon mutant deficient in glycopeptidolipid biosynthesis	26
A1	mshA	Transposon mutant deficient in mycothiol biosynthesis	19

^aKO, knockout; PIM, phosphatidylinositol mannosides; LM, lipomannan; LAM, lipoarabinomannan.